A brand-new efficient and precise high performance liquid Chromatographic Method for Simultaneous Estimation of Cidofovir and Famciclovir

Rafi Syed; Kirankumar Ch; Gidyonu Paleti

doi:10.37022/jiaps.v8i3.504

Rafi.Syed PACE Institute of Technology and Sciences (Autonomous), Ongole, AP, India
Kirankumar.Ch QIS College of Engineering and Technology (Autonomous), Ongole, AP, India
Gidyonu.Paleti PACE Institute of Technology and Sciences (Autonomous), Ongole, AP, India

DOI https://doi.org/10.37022/jiaps.v8i3.504

Abstract

The quantitative analysis of cidofovir and famciclovir using a Symmetry C-18 150x4.6mm, 3.5 column with a flow rate of 1ml/min has resulted in the development of a brand-new, effective, and exact high performance liquid chromatographic method. Acetonitrile and buffer are mixed in 60:40 ratios for the mobile stage, and the buffer is created by dissolving 1 mL of formic acid in 1 liter of HPLC water. The detection was done at a wavelength of 250 nm. After 8 minutes of running time, the Cidofovir and Famciclovir peaks were separated, with the former peak eluting after 2.8 minutes and the latter peak eluting after 6.4 minutes, respectively.The proposed method displays strong linearity in the concentration ranges of 7.5 g/ml to 112.5 g/ml for cidofovir and 25 g/ml to 375 g/ml for famciclovir. The findings of the precision and recovery examinations range from 98 to 102%. The percent RSD is less than 2.0% in any robustness scenario. Under pressure, deterioration has little impact, and solutions remain effective for the entire day. The parameters of precision, accuracy, specificity, stability, robustness, linearity, limit of detection, and limit of quantification were evaluated and found to be within the acceptable range when the method was devised and validated in accordance with ICH guidelines.

Keywords: Simultaneous determination, Validation, ICH Guide lines, RP-HPLC, Cidofovir, Famciclovir

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Author Biographies

Rafi.Syed, PACE Institute of Technology and Sciences (Autonomous), Ongole, AP, India

PACE Institute of Technology and Sciences (Autonomous), Ongole, AP, India

Kirankumar.Ch, QIS College of Engineering and Technology (Autonomous), Ongole, AP, India

QIS College of Engineering and Technology (Autonomous), Ongole, AP, India

Gidyonu.Paleti, PACE Institute of Technology and Sciences (Autonomous), Ongole, AP, India

PACE Institute of Technology and Sciences (Autonomous), Ongole, AP, India

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