A Review on Comparison of HPLC and HPTLC
Abstract
The analytical method should be sensitive, specific, fast and accurate to establish the assurance that the equipments used in manufacturing are free of the unwanted impurity, presence of which may alter the safety and efficacy of the drug product. HPLC, UPLC techniques have established their role in pharmaceutical cleaning validation. Among the modern Analytical tools HPTLC is a powerful analytical method equally suitable for qualitative and quantitative analytical tasks. HPTLC is playing an important role in today analytical world, not in competition to HPLC but as a complementary method. One of the most obvious orthogonal features of the two techniques is the primary use of reversed phases in HPLC versus unmodified silica gel in HPTLC, resulting in partition chromatography and adsorption chromatography respectively. High Performance Thin layer Chromatography (HPTLC) technique is a sophisticated and automated form of the thin-layer chromatography (TLC) with better and advanced separation efficiency and detection limits.
Keywords:
HPTLC, HPLC, Principles, instrumentations, applications, Quality control
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References
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10. Bui, Q.; Sherma, J.; Hines, J. Using High Performance Thin Layer Chromatography-Densitometry to Study the Influence of the Prion [RNQ+] and Its Determinant Prion Protein Rnq1 on Yeast Lipid Profiles. Separations 2018, 5, 6. [CrossRef]
11. Islam, M.K.; Sostaric, T.; Lim, L.Y.; Hammer, K.; Locher, C. Antioxidant HPTLC-DPPH Fingerprinting of Honeys and Tracking of Antioxidant Constituents upon Thermal Exposure. Foods 2021, 10, 357. [CrossRef]
12. Binert-Kusztal, Z.; Starek, M.; ˙ Zandarek, J.; D ˛abrowska, M. Development of TLC Chromatographic-Densitometric Procedure for ˙ Qualitative and Quantitative Analysis of Ceftobiprole. Processes 2021, 9, 708. [CrossRef]
13. Raja, T., Rao, A.L., (2012). Validated HPTLC method for simultaneous quantitation of paracetamol and lornoxicam in bulk drug and pharmaceutical formulation.Int J Pharm Biomed Res. 3 (3):162-166.
14. T. E., Barnard, J., & Cunningham, D. G. 1984. A procedure for the sensory analysis of gas chromatographic effluents.Food Chemistry, 14(4), pp.273-286.
15. Kakde,R.,Gadpayale, K., Qureshi, M.O., (2012). Stability Indicating HPTLC Method for Determination of Moxonidine in Pharmaceutical Preparations.Int J PharmTech Res. 4(1):358-363.
16. Marcin, K., Michal, M., Wiktor, S., Martyna, T., Adam, B., (2012). Application of HPTLC and LC-MS Methods for Determination of Topiramate in Pharmaceutical Formulations.Curr Pharma Anal. 8(1):44-48
17. Raja, T., Rao, A.L., (2012). Validated HPTLC method for simultaneous quantitation of paracetamol and lornoxicam in bulk drug and pharmaceutical formulation.Int J Pharm Biomed Res. 3 (3):162-166.
18. Sahoo, M., Syal, P., Hable, A.A., Raut, R.P., Choudhari, V.P., Kuchekar, B.S.,(2011). Development and validation of a HPTLC method for simultaneous estimation of lornoxicam and thiocolchicoside in combined dosage form. Pharm Methods. 2(3):178-183.
19. Sanganalmath, P.U., Purigali, M., Bannur, M.M., (2013). HPTLC method for the assay of thiopental in post-mortem blood in a fatal case of suicide.J Pharm and Biomedical Anal.80:89– 93.
20. Koba, Marcin, Marszał, Michał, P., Sroka, (2013). Determination of lamotrigine in tablets using HPTLC, HPLC, and derivative spectrophotometry methods. J lqchrrel tech. 36(4):537-548.
2. Variyar, P.S.; Chatterjee, S.; Sharma, A. Fundamentals and Theory of HPTLC-Based Separation. In High-Performance Thin-Layer Chromatography (HPTLC); Srivastava, M., Ed.; Springer: Berlin/Heidelberg, Germany, 2011; pp. 27–39. ISBN 978-3-642-14024-2.
3. Patel, R.B.; Patel, M.R.; Batel, B.G. Experimental Aspects and Implementation of HPTLC. In High-Performance Thin-Layer Chromatography (HPTLC); Srivastava, M., Ed.; Springer: Berlin/Heidelberg, Germany, 2011; pp. 41–54. ISBN 978-3-642-14024-2.
4. Charegaonkar, D. High-Performance Thin-Layer Chromatography: Excellent Automation. In High-Performance Thin-Layer Chromatography (HPTLC); Srivastava, M., Ed.; Springer: Berlin/Heidelberg, Germany, 2011; pp. 55–65. ISBN 978-3-642-14024-2.
5. Parys, W.; Pyka-Paj ˛ak, A.; Dołowy, M. Application of Thin-Layer Chromatography in Combination with Densitometry for the Determination of Diclofenac in Enteric Coated Tablets. Pharmaceuticals 2019, 12, 183. [CrossRef] [PubMed]
6. Fried, B.; Reddy, A. Recent High Performance Thin Layer Chromatographic Studies on BiomphalariaGlabrata (Gastropoda). Chromatography 2015, 2, 118–124. [CrossRef]
7. Choma, I.; Jesionek, W. TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants. Chromatography 2015, 2, 225–238. [CrossRef]
8. Orfali, R.; Perveen, S.; Aati, H.Y.; Alam, P.; Noman, O.M.; Palacios, J.; Al-Kurbi, B.S.S.; Al-Taweel, A.M.; Khan, A.; Mehmood, R.; et al. High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in PeriplocaAphylla Extracts. Separations 2021, 8, 44. [CrossRef]
9. Noman, O.M.; Nasr, F.A.; Mothana, R.A.; Alqahtani, A.S.; Qamar, W.; Al-Mishari, A.A.; Al-Rehaily, A.J.; Siddiqui, N.A.; Alam, P.; Almarfadi, O.M. Isolation, Characterization, and HPTLC-Quantification of Compounds with Anticancer Potential from LoranthusAcaciaeZucc. Separations 2020, 7, 43. [CrossRef]
10. Bui, Q.; Sherma, J.; Hines, J. Using High Performance Thin Layer Chromatography-Densitometry to Study the Influence of the Prion [RNQ+] and Its Determinant Prion Protein Rnq1 on Yeast Lipid Profiles. Separations 2018, 5, 6. [CrossRef]
11. Islam, M.K.; Sostaric, T.; Lim, L.Y.; Hammer, K.; Locher, C. Antioxidant HPTLC-DPPH Fingerprinting of Honeys and Tracking of Antioxidant Constituents upon Thermal Exposure. Foods 2021, 10, 357. [CrossRef]
12. Binert-Kusztal, Z.; Starek, M.; ˙ Zandarek, J.; D ˛abrowska, M. Development of TLC Chromatographic-Densitometric Procedure for ˙ Qualitative and Quantitative Analysis of Ceftobiprole. Processes 2021, 9, 708. [CrossRef]
13. Raja, T., Rao, A.L., (2012). Validated HPTLC method for simultaneous quantitation of paracetamol and lornoxicam in bulk drug and pharmaceutical formulation.Int J Pharm Biomed Res. 3 (3):162-166.
14. T. E., Barnard, J., & Cunningham, D. G. 1984. A procedure for the sensory analysis of gas chromatographic effluents.Food Chemistry, 14(4), pp.273-286.
15. Kakde,R.,Gadpayale, K., Qureshi, M.O., (2012). Stability Indicating HPTLC Method for Determination of Moxonidine in Pharmaceutical Preparations.Int J PharmTech Res. 4(1):358-363.
16. Marcin, K., Michal, M., Wiktor, S., Martyna, T., Adam, B., (2012). Application of HPTLC and LC-MS Methods for Determination of Topiramate in Pharmaceutical Formulations.Curr Pharma Anal. 8(1):44-48
17. Raja, T., Rao, A.L., (2012). Validated HPTLC method for simultaneous quantitation of paracetamol and lornoxicam in bulk drug and pharmaceutical formulation.Int J Pharm Biomed Res. 3 (3):162-166.
18. Sahoo, M., Syal, P., Hable, A.A., Raut, R.P., Choudhari, V.P., Kuchekar, B.S.,(2011). Development and validation of a HPTLC method for simultaneous estimation of lornoxicam and thiocolchicoside in combined dosage form. Pharm Methods. 2(3):178-183.
19. Sanganalmath, P.U., Purigali, M., Bannur, M.M., (2013). HPTLC method for the assay of thiopental in post-mortem blood in a fatal case of suicide.J Pharm and Biomedical Anal.80:89– 93.
20. Koba, Marcin, Marszał, Michał, P., Sroka, (2013). Determination of lamotrigine in tablets using HPTLC, HPLC, and derivative spectrophotometry methods. J lqchrrel tech. 36(4):537-548.
Published
20/09/2023
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Badri, S., J, R. K., P, S., M, P., K, B., E, A., & B, L. (2023). A Review on Comparison of HPLC and HPTLC. Journal of Innovations in Applied Pharmaceutical Science (JIAPS), 8(3), 18-23. https://doi.org/10.37022/jiaps.v8i3.491
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