Pancreatic protective effect of ethanolic extract of Mengkudu (Morinda citrifolia) on rats induced by doxorubicin

Elysabet; I. Nyoman Ehrich Lister; Edy Fachrial; Shahna; Sukirman Lie

Elysabet Faculty of Medicine, Universitas Prima Indonesia, Medan, Sumatera Utara, Indonesia.
I. Nyoman Ehrich Lister Faculty of Medicine, Universitas Prima Indonesia, Medan, Sumatera Utara, Indonesia.
Edy Fachrial Faculty of Medicine, Universitas Prima Indonesia, Medan, Sumatera Utara, Indonesia.
Shahna Faculty of Medicine, Universitas Prima Indonesia, Medan, Sumatera Utara, Indonesia.
Sukirman Lie Faculty of Medicine, Universitas Prima Indonesia, Medan, Sumatera Utara, Indonesia.

Abstract

Cancer is a disease or abnormality in the body due to body cells that grow and develop abnormally and beyond normal and very wild limits and doxorubicin is used in cancer therapy, but produces reactive oxygen species (ROS) which are toxic to pancreatic beta cells and made pancreatic not properly produced insulin can causes increasing of glucose level. The purpose of this study was to determine the protective pancreatic activity of mengkudu fruit ethanol extract against rats induced with doxorubicin. Mengkudu fruit ethanol extract was obtained by maceration. The protective pancreatic activity test is carried out by measuring blood glucose and blood HbA1c levels. Animals were induced with doxorubicin (DOX) 5 mg/kgbw on day 1, 7, 14 and 20th. and blood glucose is checked on the first day before doxorubicin-induced and afterward on days 5, 10, 15, and 20. Administration of mengkudu extract 100 mg/kgbw, 300 mg/kgbw, and 500 mg/kgbw given starting from day 1 to day 20 and on the 21st-day blood was taken then HbA1c levels were examined. The results obtained of ethanol extract of mengkudu fruit dose of 100 mg/kgbw, 300 mg/kgbw, and 500 mg/kgbw had a significant decrease in blood glucose levels (P <0.05) with a negative control group that was only given CMC-Na and doxorubicin. Ethanol extract of mengkudu fruit dose of 100 mg/kgbw, 300 mg/kgbw, and 500 mg/kgbw had a significantly reduced activity of HbA1c levels (P <0.05) with a negative control group that was only given CMC-Na and doxorubicin. Enhancement mengkudu does increase in rats given doxorubicin resulted in improved pancreatic beta cells and decreased blood glucose and HbA1c.

Keywords: Blood glucose, HbA1c, Doxorubicin, Mengkudu

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