Phytochemical and pharmacological evaluation of Abelmoschus esculentus (Lady’s Finger) extracts for antidiabetic activity

  • Kalyani Kulkarni C.U. Shah College of Pharmacy, S.N.D.T. Women’s University, Sir Vithaldas Vidyavihar, Juhu Tara Road, Santacruz (W) Mumbai-400049, India.

Abstract

The present investigation was aimed to study antidiabetic activity of Abelmoschus esculentus extracts in streptozotocin (STZ) induced rat model. Extracts of Abelmoschus esculentus were subjected to qualitative tests for presence of various phytochemical constituents. TLC and HPTLC studies of the extracts were carried out to identify various phytoconstituents present in the extracts. Further, acute toxicity study was carried out by administering the test solutions orally to rats, at the dose level of 2000 mg/kg for 14 days, to check whether the test solution had any toxic effects. Experimental diabetes mellitus was induced by injecting streptozotocin in normal adult male wistar rats. Intra-peritoneal injection of 60mg/kg dose of streptozotocin in adult wistar male rats, made pancreas swell and caused degeneration in islet of Langerhans and induced experimental diabetes mellitus in 2-4 days. Blood glucose levels and body weights were estimated on 0th, 7th, 14th, 21st and 28th day of the study whereas, various biochemical parameters like SGOT, SGPT, cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, urea, creatinine and total protein were estimated on 0th, 14th and 28th day of the study. There was a significant decrease in the blood glucose levels and a significant increase in the body weights of rats when compared to diabetic rats group. Also, all the biochemical parameters showed a significant decrease in their levels at the end of the study.

Keywords: Abelmoschus esculentus, Streptozotocin, SGPT, SGOT

Downloads

Download data is not yet available.

References

1. https://www.staff.ncl.ac.uk/philip.home/who dmg.pdf
2. Jarald E, Joshi SB and Jain DC. Diabetes and Herbal Medicine. Iranian Journal of Pharmacology and Therapeutics 2008; 7:97-106.
3. Glis H, Wiklund I. Nutritional Properties of Abelmoschus Esculentus as Remedy to Manage Diabetes Mellitus: A Literature Review, 2011 International Conference on Biomedical Engineering and Technology IPCBEE; l. (11), (2011).
4. Ozougwu, J. C., Obimba, K. C., Belonwu, C. D., and Unakalamba, C. B., The pathogenesis and pathophysiology of type 1 and type 2 diabetes mellitus. Journal of Physiology and Pathophysiology 2013; 4(4): 151-159.
5. Global report on diabetes, World Health Organization, ISBN 978 92 4 156525 7 (NLM classification: WK 810).
6. Indah Mohd Amin, Universiti Teknologi MARA (UiTM) Malaysia, Nutritional Properties of Abelmoschus Esculentus as Remedy to Manage Diabetes Mellitus: A Literature Review, 2011 International Conference on Biomedical Engineering and Technology IPCBEE. vol.11, 2011.
7. Saha D, Jain B, Jain VK. Phytochemical evaluation and characterization of hypoglycemic activity of various extracts of Abelmoschus esculentus Linn. International Journal of Pharmacy and Pharmaceutical Sciences 2011; 3:(2):183-185.
8. Khandelwal KR: Practical Pharmacognosy techniques and experiments. Nirali Prakashan, Second edition 2009.
9. Kumar SD, Tony DE, Kumar AP, Ashok KK, Rao SBD, Ramarao N. A review on Abelmoschus esculentus. International Research Journal of Pharmaceutical and Applied Sciences 2013; 3(4):129-132.
10. Kloppel G, Lohr M, Habich K, Oberholzer M, Heitz PU. Islet pathology and the pathogenesis of type 1 and type 2 diabetes mellitus revisited 1985; 4:110-125.
11. Broadhrust CL, Polansky MM, Anderson RA. Insulin- like biological activity of culinary and medicinal plant extracts in vitro. J Agro Food Chem. 2000; 48(3):849-852.
12. D. C. Nieman, Dr. P. H, FACSM ACSM’S Health and Fitness Journal 2005.
13. Catherine CC. Diabetes-Vital Statistics. USA: American Diabetes Association; 1996:65-80.
14. Lucy et al. Alternative therapies for Type II Diabetes. Alternative Medicine Review 2002; 7:45-58.
15. Harris MI, Diabetes in America, National Institute of Health, National Institute of Diabetes and Digestive and kidney Disease. NIH publication, 1995; 95.
16. Nidhiya ISR, P. Tatke. Phytochemical and Pharmacological Investigation of some Plants for Antidiabetic Activity, Ph.D. thesis, submitted to S.N.D.T Women’s University.
17. Chopra RN, Nayer SL. Glossary of Indian Medicinal Plants, CSIR, New Delhi, 1956.
18. Leahy JL. Natural History of beta cell dysfunction in NIDDM, Diabetes Care 1990; 13: 992-1010.
19. Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and Extraction: A Review. International Pharmaceutica Sciencia 2011; 1: 88-110.
Published
31/12/2017
Statistics
163 Views | 83 Downloads
Citatons
How to Cite
Kulkarni, K. (2017). Phytochemical and pharmacological evaluation of Abelmoschus esculentus (Lady’s Finger) extracts for antidiabetic activity. Journal of Innovations in Applied Pharmaceutical Science (JIAPS), 2(4), 05-17. Retrieved from https://saap.org.in/journals/index.php/jiaps/article/view/192
Section
Research Article(S)